Jan’s Publications

@article{WOS:000186722000004,

title = {Different levels of natural antibodies in chickens divergently selected 

 for specific antibody responses},

author = {HK Parmentier and A Lammers and JJ Hoekman and GD Reilingh and ITA Zaanen and HFJ Savelkoul},

doi = {10.1016/S0145-305X(03)00087-9},

issn = {0145-305X},

year  = {2004},

date = {2004-01-01},

journal = {DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY},

volume = {28},

number = {1},

pages = {39-49},

publisher = {ELSEVIER SCI LTD},

address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND},

abstract = {We studied the presence of Natural antibodies in plasma samples from 

 individual birds from selected chicken lines at young and old age. 

 Binding, specificity, and relative affinity to various antigens were 

 determined in plasma from non-immunized female chickens at 5 weeks of 

 age, and in plasma obtained from the same chickens one year later using 

 indirect two-step ELISA. Birds were from three different lines. The 

 lines were divergently selected for either high (H line) or low (L line) 

 antibody titers to Sheep Red Blood Cells at 5 weeks of age, next to a 

 random bred control (C line). 

 Binding of plasma immunoglobulins (Ig) from all three lines was found 

 with chicken-egg-white protein (CEP), ovalbumin (OVA), myoglobin (MYO), 

 thyroglobulin (THYRO), keyhole limpet hemocyanin (KLH), bovine serum 

 albumin (BSA), and transferrin (TRANS). Significantly higher binding to 

 most antigens was found with plasma Ig from adult birds from the H line 

 as compared to plasma Ig from the L line, whereas binding of plasma Ig 

 from C-line birds was in between or similar to the H or L line, 

 respectively. Binding of Ig to all antigens in all three lines was 

 significantly higher in plasma obtained at one year of age as compared 

 to plasma obtained at 5 weeks of age. A competitive ELISA with 

 homologous and heterologous antigens was used for determining 

 specificity of the antigen-binding antibodies. Naive plasma samples were 

 characterized by a broad binding to all antigens tested. Inhibition of 

 binding to specific antigens was possible with a broad range of 

 heterologous antigens, but highest competition of binding was obtained 

 with homologous antigen. Both linear regression analysis of serial 

 dilutions of the plasma Ig binding the antigens, as well as competitive 

 ELISA with homologous antigen indicated that plasma Ig from the H line 

 and plasma Ig from the L line had similar affinity characteristics to 

 the antigens tested with the exception of OVA and KLH. 

 Pooled non-immune plasma from H line birds bound to CEP, OVA, THYRO, 

 TRANS, MYO, KLH, and salt-precipitated extracts and supernatants of 

 extracts from chicken heart, spleen, liver, brain, bursa, thymus, and 

 kidney, respectively, as determined by Western blotting. The increasing 

 presence of antibodies in naive chicken plasma binding heterologous and 

 homologous (tissue) antigens indicates the presence of Natural 

 antibodies in poultry. Apart from age, increasing levels of Natural 

 antibodies may be related with the genetically based magnitude of 

 specific antibody levels in the chicken lines studied. (C) 2003 Elsevier 

 Ltd. All rights reserved.},

keywords = {chicken; natural antibodies; selection; age},

pubstate = {published},

tppubtype = {article}

}